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Among the 97 differentially expressed proteins at V1V0, 51 proteins were upregulated, including 28 classified as secreted to blood and 23 as leakage proteins based on human protein atlas

Nat Cell Biol 2008

M., Carr, A

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Tissue levels can quickly become depleted, such as with an overnight or extended fast
After 5 weeks, mice in the KBD group showed symptoms of biotin deficiency, including hair loss and dermatitis