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Description
Similar to the human GCLC, up-regulation of the human GCLM by -NF involved binding of transcription factor Nrf2 (possibly in complexes with other Jun or Maf proteins as in GCLC) to a functional ARE/EpRE site located at 302 of the human GCLM [104,134]
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2.8 16S rRNA sequencing and analysis In order to evaluate the differences in the intestinal flora between the treatment group and the AD group and the control group, three samples were collected from each group, each sample had 15 intestines, and the genome of the bacterial colony was extracted from the intestinal contents for amplification, and the recovered products were amplified using fluorescence quantitative polymerase chain reaction
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The supernatant was removed and the pellet was washed by adding 800 l pre-cooled (-20 o C) acetone
However, NAC as add-on therapy in nondiabetic patients with chronic kidney disease who were on renin-angiotensin system blockade medication had no effect on blood pressure [78]
