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A 2030 g of total protein were resolved on 412% NuPAGE BisTris gels (Thermofisher) and transferred to nitrocellulose membrane using a semi-dry iBlot2 kit (Thermofisher)

This deficiency may lead to amino acid misincorporation and/or truncation of the polypeptide, thus affecting the heterologous protein expression levels (which will be low at best) and/or its activity (Gustafsson et al., 2004)

Sci Total Environ 852:158393