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Historically, NMR spectroscopy primarily targeted the structure determination of globular proteins with regular secondary structure motifs, following the path laid by X-ray crystallography

This work was also supported by the Basic Research Lab Program (2022R1A4A1025557) funded by the Ministry of Science and ICT and the Korean Fund for Regenerative Medicine (KFRM) grant (the Ministry of Science and ICT, the Ministry of Health & Welfare) No

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Studies were excluded if they did not provide integrated data or did not have an appropriate intervention

It is important to check for precipitation of the purified protein during the dialysis

Understanding the Core Mechanisms: What Are We Looking At
