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Tanshinone IIA can inhibit focal death by modulating Txnip/NLRP3 inflammatory vesicles, regulation of TGFB1 inhibits high glucose-induced inflammation and cellular pyroptosis in renal tubular epithelial cells, may promote defense against DKD by inhibiting apoptosis caused by intracellular oxidative stress, can prevent podocyte damage in DKD by promoting autophagy and inhibiting inflammation, in part by inhibiting the PI3K/Akt/mTOR signaling pathway (Li et al., 2024), also ameliorates DKD-induced renal fibrosis by regulating miRNA-34a-5p in in vitro and in vivo studies

8 L of a 25 mM DMSO solution of CysOx1 or CysOx2 was added, followed by 3.0 L of 1 mM H 2 O 2 or 700 mM TCEP in ddH 2 O

Within a minute or two, you should have a completely clear solution

Therefore, the purified PEGylated protein from a mass ratio of PEG:protein as 1:3 is used in this study

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