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In summary, the roGFP2 oxidation kinetics of PfGrx fusion constructs reflect at up to micromolar GSSG concentrations the glutathionylation kinetics of the glutaredoxin moiety in accordance with previous intracellular structure-function analyses and data interpretations 15,34,35

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NAC treatment provides a source of cysteine, a ratelimiting building block for GSH synthesis, and is known to increase GSH levels in the brain 46

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