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Cell lysis for protein preparation The yeast cell pellet in a 1.5-mL tube (on ice) was resuspended with 500 L ice-cold lysis buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1.8 mM MgCl 2 , 0.5% NP-40, 1 mM PMSF, 1 mM DTT, 20 M MG132, 1 tablet/10 mL cOmplete mini EDTA-free (Roche)) and transferred to a new 1.5-mL tube containing 50 L of 0.5 mm Zirconia/Slica Beads (BioSpec Products)
10.1016/j.nano.2016.02.010 191 XuC.WanX.XuL.WengH.YanM.MiaoM.et al (2015)
BrdU staining indicated significantly more proliferating cells in HG of the P3O-treated group compared with the control group (Fig
Tawfeek HM, Chavan T, Kunda NK (2020) Effect of spray drying on amorphization of indomethacin nicotinamide cocrystals
[DOI] [PubMed] [Google Scholar] 83.Kakimoto K, Kojima Y, Ishii K, Onoue K, Maeda H
Not a single commercial product exists after a century of trying
