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After 90 min, the reaction was stopped by adding DMSO, and absorbance was measured at 570 nm (Varioskan Flash Spectral Scanning Multimode Readers, Thermo Scientific, Waltham, MA, USA)

Copper and zinc dismetabolism in the mouse brain upon chronic cuprizone treatment

TJ concentrations were analyzed using ELISA

Reconstitution is the process of reintroducing a sterile liquida diluentto this powder to bring it into a stable, injectable solution ready for laboratory use

While phosphorylation levels of PI3K, protein kinase B (PKB, aka AKT), and mammalian target of rapamycin (mTOR) were significantly increased in the gastrodin-treated OLs (Fig