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Importantly, CHAC2 did not localize to the mitochondria irrespective of extracellular cystine availability, further suggesting divergent roles for these proteins in mediating intracellular GSH catabolism

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The cDNA for amplification in the PCR assay was constructed by reversion transcription reaction using Revertaid H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Waltham, USA)

Redox Signal 9 , 775806 (2007)

Consistent with this outcome, topical application of the n-3 fatty acid eicosatrienoic acid (20:3n-3) protected against UV-induced skin damage in hairless mice (46)