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Membranes were blocked with TBS (20 mM Tris-HCl [Ph 7.6], 137 mM NaCl) containing 5% non-fat dried milk and 0.05% Tween 20

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B-7 (biotin): Biotin is known for its role in maintaining healthy hair, skin, and nails, but thats not the only purpose that this B-complex vitamin serves

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Golubnitschaja O, Liskova A, Koklesova L, Samec M, Biringer K, Bsselberg D, et al