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Endpoints frequently measured include the activity of alkaline phosphatase (ALP), an early marker of osteoblast differentiation, the expression of the master osteogenic transcription factor Runx2, and the deposition of a mineralized matrix, which can be visualized with Alizarin Red staining

Interestingly, the protective effects of these antioxidants can still be noticeable ten years after the end of supplementation (160)

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[DOI] [PMC free article] [PubMed] [Google Scholar] 68.Bulboac A.E., Bolboac S.D., Stnescu I.C., Sfrngeu C.A., Bulboac A.C

J Nutritional & Environmental Medicine

Its a simple estimate based on total amount divided by your target amount per dose, rounded down