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In the case of this HCV diagnostic the primers were tagged with biotin and digoxigenin (DXN) so that when the amplified DNA was put on the LFA strip the biotin bound to streptavidin coated gold nanoparticles and then the digoxigenin bound to antibodies on the test line

10.1111/jnc.12215 147 Velasco-SnchezD.AracilA.MonteroR.MasA.JimnezL.OCallaghanM.et al (2011)