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Common causes of temporary post-injection pain include: Local inflammation: The injection itself can irritate surrounding tissues Increased joint pressure: The added fluid volume temporarily stretches the joint capsule Normal healing response: Your bodys natural reaction to any needle puncture Muscle tension: Protective guarding around the injection site According to Cleveland Clinic, this reaction typically manifests as mild pain and swelling at the injection site and resolves without intervention [3]

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The trend of nitrate transport followed by glutamine/glutamate metabolic process is more obvious in maize roots, reflecting the sequential process of nitrogen uptake and assimilation in plants (Supplementary Fig