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Fragment size was confirmed on the TapeStation 4200 using the High Sensitivity DNA kit (Agilent)

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The primary antibodies used in the study included anti-AKT (1:1,000, Affinity Biosciences, Changzhou, China), anti-p-AKT (1:1,000, Cell Signaling Technology, Shanghai, China), anti-p38 MAP kinase (1:1,000, Affinity Biosciences), anti-phosphorylated p38 (1:1,000, Cell Signaling Technology), and anti--actin (1:2,000, Sangon, Shanghai, China)