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A trial with broiler chickens conducted by Raju and Devegowda (2000) showed a bone ash-decreasing effect of AFB1 (300 g/kg), OTA (2 mg/kg), and T-2 toxin (3 mg/kg), fed individually but an incomparable higher effect when fed in combination

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and 0.8 mL of 0.2 M phosphate buffer to the final volume of 3 mL

doi: 10.1007/s11104-015-2787-8 253 YangZ