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7C) and volume (Fig
This stock solution was then diluted to 200 M using 5% BSA
Activated platelets (aPLT) were obtained by stimulating platelets with 2 U mL 1 thrombin and 8 nM CaCl 2 for 10 min at 37 C, The aPLT were then suspended in PBS containing 2% DMSO and frozen in liquid nitrogen for 12 h
In these reactions, glutathione (GSH) is used as a reducing agent (Figure 9), which is restored by the enzyme glutathione reductase
Eriksen 15 , L
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