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Tuli et al., 2013a

J Neurosci Res 11:351357 McKenna MC (2012) Substrate competition studies demonstrate oxidative metabolism of glucose, glutamate, glutamine, lactate and 3-hydroxybutyrate in cortical astrocytes from rat brain

The goal of the 3R Principle is to avoid animal experiments altogether (Replacement) and to limit the number of animals (Reduction) and their suffering (Refinement) in tests to an absolute minimum

in this situation, NAC reduces the risk of hepatotoxicity to 100 mg/L, the presence of renal failure or ARDS, or progression of the toxidrome despite alkalinization therapy (39)