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NRK (normal rat kidney) cells, was used as an example of asymptomatic because rats are known to be typically asymptomatic carriers of serovar Manilae strains 22

For serum samples, 75 L of chloroform:methanol extraction solvent (v:v = 1:2) was added to 20 L of mouse serum, with the exception of the serum from WT, Gclc L-KO, Nrf2 L-KO, and L-DKO mouse serum, for which 20 L of mouse serum was combined with 180 L of chloroform:methanol extraction solvent (v:v = 1:2) containing internal standards at the final concentrations: 5 nM D7-Sphinganine (Avanti Polar Lipids Inc., Cat# 860658), 12.5 nM D3-Deoxysphinganine (Avanti Polar Lipids Inc., Cat# 860474), and SPLASH LIPIDOMIX (1:1000, Avanti Polar Lipids Inc., Cat# 330707)

In this case, do not add Coenzyme working solution or Enzyme working solution, Buffer solution and pure water are added

Glutathione is often referred to as the bodys master antioxidant and detoxifier for cells

doi: 10.1063/5.0013178, 170