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Oncol Res (2017) 25:30516

The ratio of the luminescence signal measured at 535nm over at 485nm (Lm1/Lm2) was calculated and used as BRET values

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However, there are reagents that will react with carboxylic acids and allow them to be activated for ligand attachment

Kunnumakkara 2019

doi: 10.1186/s13229-021-00447-5 151 Zuiderwijk-SickE
