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Introduction As abundant, circulating, vascular guards, platelets are uniquely positioned to detect and respond to vascular damage to stop bleeding and maintain vascular homeostasis

Control culture variables that affect signaling: Passage number, confluence, serum concentration, extracellular matrix coating, oxygen condition, mycoplasma status, baseline cell health, and image-analysis thresholds can all influence FAK phosphorylation, VEGFR2 readouts, and tube-formation results

Storage and Handling BPC-157 is typically supplied in lyophilized (freeze-dried) form, allowing for stable storage prior to research use

doi: 10.1096/fj.03-0782fje

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