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3 Host-NTHi competition for haem-iron Iron is an essential micronutrient for pathogen and host alike, with critical roles in many vital cellular processes, both as an inorganic ion and through incorporation into haem, which is a molecule composed of a protoporphyrin IX (PPIX) ring and a single central iron atom (Parrow et al., 2013

Unlike standard glutathione tablets or powders, this liposomal glutathione format allows more efficient utilisation by the body, helping ensure consistent daily antioxidant support and making it a practical alternative to traditional glutathione supplements

3 Although alteration of antioxidant enzymes has been implicated in glaucomatous ONH astrocytes, 6 it remains unknown whether oxidative stress alters the mitochondrial network and triggers bioenergetic dysfunction in the ONH astrocytes

Proteins were separated by SDS-PAGE, glutathionylated proteins were visualized with anti-GSH antibodies (#ab19534, abcam, dilution 1:1000) after Western blotting

465 Ferrostatin-1 was first modified to UAMC-2418 by replacing the labile ester with amide or sulfonamide and adding a benzyl ring to NH 2

Zhitkovich, A