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The metal boot was kept on all times until euthanasia, except during skin inspection, when rats were sedated with isoflurane and the boot was open

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Ahsan, H., Ahad, A., Iqbal, J

doi: 10.1111/pbi.13116 14 BilichakA.IlnytskyyY.WycickiR.KepeshchukN.FogenD.KovalchukI

When activated, the mast cells in the skin release mediators (histamines, tryptase, TNF-alpha, CXCL9, etc.) that cause inflammation, flushing, itching, or burning.[ref] Related article: Rosacea, underlying causes, genetic susceptibility Dermatographism and mast cell activation syndrome: In a survey of mast cell activation syndrome, dermatographism was found in 89% of the patients.[ref] Dermatographism is when you get welts or raised red lines when you scratch your skin lightly

1 KCs have a dynamic response depending on the type of stimulation and can secrete various sets of cytokines and chemokines, including Interleukin (IL)1, IL20, and tumor necrosis factoralpha (TNF) that can coordinate the skin inflammatory response whenever it is triggered