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The reaction flask was sealed, covered by aluminum foil to prevent the light, taken out from the glovebox, and stirred at 40 C in an oil bath for 24 h

AMP-Activated Protein Kinase (AMPK) is a pivotal enzyme studied for its central role in cellular energy regulation, metabolic signaling, and mitochondrial function

Others stop immediately without issues

Nature Medicine, 14 (5), 504506

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Zhang HL, Ersoy H, Prince MR
