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You can mix either: 1mL (100 IU) for stronger concentration 2mL (200 IU) for gentler, more flexible dosing Both options are safe and effective your choice depends on how precise you want to be with smaller doses

Gene expression analysis with RTqPCR Gene expression was analyzed via reverse transcriptase quantitative polymerase chain reaction (RTqPCR) using TaqMan probes (Life Technologies) or SSO Universal Probes Master Mix (Bio-Rad)