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Then, cells were incubated with PE-conjugated anti-mouse CD86 or CD206 antibodies (2 L/test, BioLegend) for 30 min at 4 C in darkness

As we age, natural collagen production begins to decline, leading to visible signs of aging such as wrinkles, sagging skin, brittle nails, thinning hair, and stiff joints

1 Introduction Hepatocellular carcinoma (HCC) is the most frequent malignant tumor of the liver, with the highest occurrence among people with chronic liver disease ( Ferroptosis is an iron-dependent controlled cell death caused by polyunsaturated fatty acid-containing phospholipids (PL-PUFA) in cell membranes that differentiates from other types of cell death by iron-dependent accumulation of lipid peroxides and redox imbalance ( 2 The mechanism of ferroptosis The rise in ROS surpasses the regulating capacity of glutathione (GSH) and GPX 4, resulting in lipid peroxidation ( Figure 1 )