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10 To test whether the induction of p70S6K1 phosphorylation is due to inhibition of IGF-1R signaling pathway not non-specific outcome of OSI-906, the HCT116 cells were treated with other IGF-1R inhibitors, BMS-754807 and GSK1838705A, for 072 h

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This includes: Direct evidence: directly measured experimental support that blocking or preventing a KE prevents or impacts downstream KEs in the pathway in the expected fashion