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Measurements were made using an Agilent 7700 series ICP-MS instrument under routine multi-element operating conditions using a Helium Reaction Gas Cell

no need for HRP as a coupling enzyme Overview The ROS-Glo HO Assay is a homogeneous, fast and sensitive bioluminescent assay that measures the level of hydrogen peroxide (HO), a reactive oxygen species (ROS), directly in cell culture or in defined enzyme reactions

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The strength of this synergy depends on the reaction rates and the concentration of these antioxidants