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doi: 10.1016/0887-2333(94)00187-Y

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Heat-induced epitope retrieval (HIER) was performed in a pressure cooker for 20 min using either citrate-based buffer at pH 6.0 (VectorLab, H-3300-250) or tris-based buffer at pH 9.0 (VectorLab, H-3301-250), according to the antibody manufacturers instructions

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He has re-engineered glial-derived neurotrophic factor (GDNF) as a molecular Trojan horse