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We identify STING, NEMO, OPTN and ANKIB1 itself as substrates of ANKIB1 and show, by using ANKIB1-RING1 mut cells, that the catalytic activity of ANKIB1 enables OPTN and TBK1 recruitment, contributing substantially to IRF3 activation and type-I and type-III IFN production

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GO enrichment analysis further confirmed functional changes in processes such as carboxylic acid biosynthesis, rhythmic processes, and glucose metabolism (Supplementary Fig