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In our GST purification protocol, we suggest using 1x PBS, pH 7.4 as the binding buffer

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309 ASGPR is an endocytic receptor notably overexpressed on the hepatocyte membrane surface

IGF-1 LR3 is more potent than regular IGF-1 because of the decreased binding of Long R3 IGF-1 to IGF binding proteins, allowing it to remain active up to 120 times longer compared to native IGF-1

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doi: 10.1016/j.neuropharm.2013.05.032, [DOI] [PMC free article] [PubMed] [Google Scholar] Farsi Z., Jahn R., Woehler A