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The lysis buffer used for the purification contained 50 mM NaCl instead of 150 mM NaCl
10.3390/biology10010006 41 KuryowiczA
Meanwhile, the protons that were held on FMN and His182 are re-equilibrated to bulk solution (Fig
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Reconstitution Tips for Beginners If this is your first time reconstituting any peptide, three habits will prevent virtually every common error
