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The strains N2, OH15876 ( pha-4::3xGAS::GFP::TEV::LoxP::3xFLAG ), AQ866 (ser-4 KO), DA1814 (ser-1 KO) and DV3208 ( daf-15::mNG::2xHA ) were cultivated using standard techniques (Brenner, 1974)

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Nikon) and a 50-m pinhole (0.8 m optical section thickness) or a Zeiss confocal microscope (LSM900, Axio Observer) using the 63 oil objective (Plan-Apochromat 63/1.40 oil DIC M27), a 1 Airy Unit/45 m pinhole (0.5 m optical section thickness) and the Zen software (3.0, blue)

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Curtis, R., O'Connor, G