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Description
To minimize the artefactual oxidation, alkylating reagents such as Iodoacetamide (IAM) or N -ethylmaleimide (NEM) are added at the first step during protein denaturation to alkylate reduced Cys residues (McDonagh et al., 2014)

The tetanus toxoid vaccine contains an inactivated form of the tetanus toxin

Yamazaki Y, Okawa K, Yano T et al (2008) Optimized proteomic analysis on gels of cell-cell adhering junctional membrane proteins

If the low energy was already severe before the medication, that points another

[DOI] [PubMed] [Google Scholar] [29].Gupta SK, Shukla VK, Vaidya MP, et al

Chekmeneva E
