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At the joint microenvironment level, analyses of synovial fluid and cartilage tissue samples from OA patients, as well as studies in surgically induced such as the DMM model or chemically induced animal OA models, consistently observe clear iron metabolism abnormalities and significant iron accumulation locally in affected joints

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DAPI was not used to stain the nuclei since its adsorption to the transwell filter background impairs the visualization of VE-Cad- Alexa Fluor 488 signal

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