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mTOR and AMPK phosphorylation states reflect mitochondrial vs glycolytic metabolic programs in DC and increased pmTOR:pAMPK ratio impacts metabolic alterations of tol-DCs To gain additional insights into signaling cascades regulating inflammatory and tolerogenic DC metabolism, we employed antibodies recognizing the total and phosphorylated forms of AMPK (Thr-183/172) and p-mTOR (Ser-2448) (Supplementary Fig

Therefore, it is possible that GSH homeostasis is a regulator of metabolite-induced proteotoxicity

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The filtered mixture was then dialyzed against PBS (pH 7.4) at 15 C for 12 h, ensuring the effective removal of DMSO and any unreacted polymer

Through integrated proteomic and metabolomic analyses, we identified a high-inflammation, high-oxidation, low-metabolism triad in the hippocampus of CLP-induced SAE mice, providing novel insights into mechanisms underlying persistent cognitive deficits in SAE survivors

Proper dosage and monitoring are crucial for safe and effective use