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Tsao CT, Kievit FM, Ravanpay A, Erickson AE, Jensen MC (2014) Thermoreversible poly(ethylene glycol)-g-chitosan hydrogel as a therapeutic T lymphocyte depot for localized glioblastoma immunotherapy

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2.28 Cell apoptosis analysis Chondrocytes were incubated with corresponding conditioned medium for 24 h and collected using trypsin, followed by resuspended in 500 L of binding buffer

The dried residue was reconstituted by adding 300 L of methanol, vortexed for 1 min, and centrifuged at 14,000 rpm/min, and then 4 L of the supernatant was analyzed by LC-MS

doi: 10.1023/a:1013603331899