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Excess glucocorticoids (GCs) can induce neuronal damage in brain regions such as the hippocampus and SN through several mechanisms, including mitochondrial dysfunction, inhibition of inhibitory and excitatory synaptic transmission, and disruption of microglial or astrocytic functions, thereby exacerbating the progression of PD ( 2.3 The close relationship between intestinal microbiota and PD In the 1980s, the first reports of Lewy body pathology in the ENS provided evidence for ENS and gut microbiota dysfunction in PD ( Lachnospira and Butyricicoccus ) and butyrate-producing [Eubacterium]_ventriosum_ group ( Proteobacteria, Verrucomicrobia, and Actinobacteria ) and five genera ( Akkermansia , Enterococcus , Hungatella , and two Ruminococcaceae ) in their gut microbiome

In the head, midgut and abdomen, 3 samples of 6 drones each were analyzed per hive (n = 12 for each modality)

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