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J Clin Neurosci (2019) 63:136

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One such sensor, the protein encoded by the silent information regulator2 ( SIR2 ) gene in yeast (and its mammalian orthologue SIRT1 ), mediates transcriptional silencing through its nicotinamide adenine dinucleotide (NAD)-dependent histone deacetylase (HDAC) activity

Ca 2+ increases were time delayed because drugs were pipetted into a stationary bath rather than a flow through system

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