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Neuroprotection by urate on the mutant hSOD1-related cellular and Drosophila models of amyotrophic lateral sclerosis: implication for GSH synthesis via activating Akt/GSK3/Nrf2/GCLC pathways

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The side effects observed were [31]: Nausea (24% of participants) Diarrhea (21%) Vomiting (14%) Constipation (8%) Dyspepsia (7%) Injection site reactions (3%) Cholelithiasis (less than 1%) Pancreatitis (less than 1%) Gastrointestinal side effects were the most common reason for discontinuation and their severity varied from mild to moderate

This was further validated through immunoblotting assays that confirmed the downregulation of CBS, GCLC, and GCLM in shDDX3X A549 cells (Fig