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After the membrane was blocked with 5% skim milk TBST (20 mM Tris-Cl (pH 7.4), 150 mM NaCl, 0.1% Tween-20), it was incubated with streptavidin (SA) coupled to horseradish peroxidase (HRP) (1:1000, Pierce) and washed three times with TBST for 10 min

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The Lancet Neurology 2016, 15, (7), 673-684, 10.1016/s1474-4422(16)00070-3

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PEG-uricase in the management of treatment-resistant gout and hyperuricemia

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