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In this respect, it is important to discriminate between tumors that express CA IX as a consequence of the inactivating mutation of pVHL tumor suppressor protein and tumors in which CA IX is present in connection with microenvironmental hypoxia and/or acidosis

The hereromeric GluK2/K5em receptor was expressed in HEK293S GnTI cells using the BacMam system, solubilized in 100 mM dodecyl--D-maltoside (DDM, Anatrace), 0.5% cholesterol hemisuccinate (CHS, Anatrace), followed by sequential Twin-Strep (GluK2) and 1D4 (GluK5) affinity purification and Superose 6 size-exclusion chromatography 23