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Always run two weeks at the beginner dose first
BMC Genomics 14:29

Small tweaks to technique changing the site, spacing injections differently, or modest shifts in needle angle can reduce repeated soreness

Colla, R., Izzotti, A., De Ciucis, C., Fenoglio, D., Ravera, S., Speciale, A

Compared with serum-containing medium, defined xeno-free medium increased MSC proliferation, CFU-F, and gene expression related to MSC stemness
