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Explore key factors involved in varicose vein diagnosis, including increased pressure and venous transport capacity impacts

Decades of clinical data confirm that

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Finally, after washing 3 times in PBT for 30 min each at room temperature and two times in alkaline-phosphatase (AP) buffer (100 mM Tris-HCl, pH 9.5, 100 mM NaCl, 0.1% Tween20, 50 mM MgCl 2 ) for 5 min each at room temperature, the slides were incubated with 1 L 75 mg/mL NBT and 3.5 L 50 mg/mL BCIP per mL AP buffer and developed in the dark for 2-20 h

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