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PMCID: PMC10665711

If your vial will be used more than once over days or weeks, bacteriostatic water is required

(Use undiluted extract for GSSG testing below or dilute extract 1/20 before testing for "total GSH" according to standard procedure, page 8 of NWK-GSH01 product insert

Protein pellets were washed twice with acetone and resuspended in 2X Laemmli buffer (2% SDS, 10% glycerol, 0.02% bromophenol blue, 0.05 M Tris-HCl pH 6.8, 5% 2-mercaptoethanol) for analysis by SDS-PAGE and western blot

Second, snRNA-seq analysis in this study is only suggestive because enzyme activity is regulated not only by transcription levels but also by covalent modification, such as phosphorylation and acetylation

The washout periods between sessions were at least 7 days