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Regular stability testing ensures consistent results across multiple experiments

The NAPstar3b variant was generated by site-directed mutagenesis on the pDONR207 NAPstar3 plasmid using the primer pair Pr1/Pr2 (Supplementary Table 6)

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Buffers with pH 5, 7, 9 were prepared by adjusting the pH of PBS buffer using 0.2 mol/L HCl or NaOH solution

KEY POINTS IGF-1 can promote ageing by stimulating cell growth, division and cell death

Glutathione is predominately located in the cell, whereas a major fraction of the cellular y-glutamyl transpeptidase (glutathione enzyme) is on the external surface of cell membranes