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Urine samples and standards were transferred to 96-well plates, taxifolin (1 g/mL) was added as internal standard, and samples were vortexed and centrifuged at maximum speed for 2 min before LC-MS/MS analysis

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Male wild-type mice (8 weeks old) in the C57BL6/J background were kept on a 12 h light/dark cycle at controlled temperature and humidity, with standard food (4RF21, Mucedola Srl, Italy) and water provided ad libitum and environmental enrichments

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doi:10.1007/s13197-020-04357-x 97

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